Coding

Part:BBa_K4694008:Design

Designed by: Becky Davies   Group: iGEM23_Exeter   (2023-09-29)


Sp-His-AHL-Lactonase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 261
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been mutated to remove forbidden restriction sites to be compatible with BioBrick and Type IIS assembly


Source

Bacillus thuringiensis serovar kurstaki (29339) Liu 2008

References